Factors affecting the chitinase activity of Trichoderma asperellum isolated from agriculture field soils

In the present study, 20 fungal strains were isolated from tomato rhizosphere of Senegal. Of 20 strains, five showedthe chitinolytic activity on chitin agar medium. Of the five strains, NG4 showed the maximum solubilization zone.This strain was identified by preliminary biochemical and 18S rRNA sequencing analysis. Enzyme productionstarted after 3 days of incubation and maximum was observed after 5 days of incubation. Culture filtrate amendedwith 0.1% colloidal chitin was used in the production medium. The optimum conditions for maximum chitinaseactivity are – 6 days of growth and temperature of 30°C at pH 6.0. The chitinase activity was also influenced by theaddition of carbon and nitrogen sources in the production medium.

Aural foreign bodies in children in a pediatric Senegalese hospital

Introduction: aural foreign bodies (FB) are frequent in pediatric otorhinolaryngology and in pediatric emergencies. The objective of this study was to describe the socio-demographic, clinical and therapeutic aspects of aural FB in children in a pediatric hospital in Senegal. Methods: this was a four-year hospital-based descriptive study performed in the Otorhinolaryngology department of the Children's Hospital of Diamniadio, from 1st January 2013 to 31 December 2016 including all children under 15 years of age received for aural FB. The variables studied were age, gender, provenance, presenting symptom, time to consultation, the type of FB and morbidity. Results: sixty three FB were extracted. Mean age was 6 years 4 months. 52.4% of patients were under 5 years old. There was a slight female predominance (32/63). Location was in the right ear canal in 55.6%. Seventy three percent of patients presented in our office were asymptomatic. The median time to consultation was 4 days. Within the first 24 hours after insertion, 22.2% of children presented to our office. Beads were the first FB (29.68%), followed by seeds and cotton (12.5% respectively). Foreign bodies' extraction were performed in consultation for 79.4% of patients. The morbidity was 20.6%. It was primarily otitis externa (8%). Myringoplasty was performed in one patient who had sequelae of tympanic membrane perforation. Conclusion: aural FB is a common accident in under-5 year-olds. In our context there is a delayed consultation. Morbidity is similar to that of the literature

Epidemiology, clinical features and treatment of lip cancers in Senegal

Introduction. Lip cancers are uncommon among black people but not unusual. The objectives of this study were to describe the epidemiological, clinical and therapeutic aspects of lip cancers at ENT Department of National University Hospital of Fann in Dakar, Senegal. Patients and methods. We performed a retrospective and descriptive study at ENT Department of Fann teaching Hospital in Dakar, Senegal, over a period of seven years, among patients with lip cancers confirmed by the pathology. The following data were studied: age, gender, past medical history, alcohol and tobacco intake, oral hygiene, the location of the lesions and their extensions, pathological findings, TNM classification, postoperative complications and mortality. Results. 19 cases were collected. The mean age of patients was 51 years, with a sex ratio of 1.4. Bad oral hygiene was the main risk factor followed by prolonged sunlight exposure. The lower lip was the main location of the lesion (73.7%). The budding ulcerative appearance was predominant (73.68%). Squamous cell carcinoma was found in all our patients. 68.42% of our patients were classified T3-T4. 63.15% of our patients underwent surgical resection of the tumor. The most common technique of reconstruction was Estlander flap (25%). Morbidity and mortality were respectively 50% and 31.6%. The global survival rate at one and three years was 85.7%. Conclusion. Lips cancers are relatively uncommon tumors in the black people. In our context, patients consult at advanced stages, making their treatment more difficult

L'amygdalectomie et l'adénoïdectomie à l'hôpital pour enfants de Diamniadio au Sénégal : une évaluation de 3 ans

INTRODUCTION. Les indications de l'amygdalectomie et l'adénoïdectomie sont un sujet de controverse. Notre objectif était de discuter nos indications opératoires en les confrontant à la littérature tout en précisant notre particularité dans la prise en charge des patients dans un hôpital pédiatrique sénégalais. METHODES. Etude rétrospective descriptive au service d'Oto-rhino-laryngologie de l'hôpital pour Enfants de Diamniadio du 1er janvier 2013 au 31 décembre 2015 incluant tous les patients de moins de 15 ans opérés d'une adénoïdectomie, d'une amygdalectomie ou d'une adéno-amygdalectomie. Les variables étudiées étaient l'âge, le sexe, l'indication opératoire, le type de chirurgie et la morbidité. RESULTATS. 522 patients ont été inclus. L'âge moyen était de 4 ans 9 mois avec des extrêmes de 4 mois et 15 ans. L'obstruction respiratoire chronique était la première indication opératoire (63%). Elle était dominée par le syndrome d'apnée obstructive du sommeil retrouvée chez 264 patients (50,6%). Avant l'âge de 10 ans, les indications opératoires étaient dominées par l'obstruction respiratoire chronique (68,2%). Après 10 ans, les infections prédominaient (81,5%). L'adéno-amygdalectomie était le geste chirurgical le plus réalisé (43,7%). La morbidité était de 0,6%, représentée exclusivement par un cas d'hémorragie postopératoire immédiate et deux cas d'hémorragie secondaire.CONCLUSION. L'obstruction respiratoire chronique est la principale indication opératoire particulièrement avant l'âge de 10 ans. Les complications postopératoires sont rares